Hepatocellular Carcinoma Recombinant Antigen-Antibody Detection
Restalyst HCC-REAAD™ ERBB3 ELISA is an
in-vitro diagnostic intended for the qualitative
and semi-quantitative detection of
Receptor tyrosine-kinase erbB-3 (also
known as HER-3) in human serum/plasma
for early detection of hepatocellular
carcinoma. ERBB3 protein (patented) is
discovered to have elevated concentration
levels in the serum/plasma of hepatocellular
carcinoma patients.
A new Algorithm (binary logistics regression) incorporating the use of ERBB3 and existing marker AFP improves the efficancy of diagnosis which is lacking in the conventional routine screening using AFP alone.
Capture antibodies specific to ERBB3 protein are coated on the microwell. ERBB3 proteins present in the human serum/ plasma will form antigen-antibody complexes with the capture antibodies. Biotinylated detection antibodies are introduced for the coupling with complexes. Secondary antibodies conjugated with horseradish peroxidase (HRP) are added to bind to the detection antibodies. A substrate solution (TMB) is added and is converted by the enzyme to a detectable form (color signal). The intensity of this colored product is directly proportional to the concentration of antigen present in the original specimen. Microwells can be read on any suitable spectrophotometer or ELISA reader.