Gastric Carcinoma Recombinant Antigen-Antibody Detection
Restalyst GC-REAAD™ ITIH3 ELISA is an
in-vitro diagnostic intended for the qualitative
and semi-quantitative detection of
Inter-alpha-trypsin inhibitor heavy chain H3
(ITIH3) protein in human plasma for early
detection of gastric carcinoma. ITIH3
protein, a patented technology, was found
to be expressed at high levels in the plasma
of gastric carcinoma patients which is not
a ected by the gastritis status or H.pylori
infection.
GC-REAAD™ determines the patients’ risk level of gastric carcinoma, either positive or negative, by using an arbitrary unit of measurement known as REAAD™-units (RU).
Capture antibodies specific to ITIH3 protein are coated on the microwell. ITIH3 proteins present in the human plasma will form antigen-antibody complexes with the capture antibodies. Detection antibodies are introduced for the coupling with complexes. Secondary antibodies conjugated with horseradish peroxidase (HRP) are added to bind to the detection antibodies. A substrate solution (TMB) is added and is converted by the enzyme to a detectable form (color signal). The intensity of this colored product is directly proportional to the concentration of antigen present in the original specimen. Microwells can be read on any suitable spectrophotometer or ELISA reader.